Cloning and expression analysis of the chitinase gene Chit1 from entomopathogenic fungus Nomuraea rileyi CQ strain
莱氏野村菌Cq菌株几丁质酶基因的克隆与表达分析

ObjectiveIn order to understand the role of the entomopathogenic fungal chitinase in invasion and pathogenic process,we cloned and expressed a chitinase gene from Nomuraea rileyi CQ strain,and detected chitinase activity in recombinant Pichia pastori fermentation supernatant.MethodsSpecific primers were used to amplify the complete sequence of chitinase gene and open reading frame(ORF) from genomic DNA of N.rileyi Cq Strain extracted by CTAB.The fragment of ORF was linked with vector plasmid pPIC9K to const...