%0 Journal Article
%T Cloning and expression analysis of the chitinase gene Chit1 from entomopathogenic fungus Nomuraea rileyi CQ strain<br>莱氏野村菌Cq菌株几丁质酶基因的克隆与表达分析
%A Jinfang Wang
%A Guangying Liu
%A Yi Zhai
%A Yongji Wan
%A <br>王金芳
%A 刘光英
%A 翟逸
%A 万永继
%J 微生物学报
%D 2010
%I 
%X ObjectiveIn order to understand the role of the entomopathogenic fungal chitinase in invasion and pathogenic process,we cloned and expressed a chitinase gene from Nomuraea rileyi CQ strain,and detected chitinase activity in recombinant Pichia pastori fermentation supernatant.MethodsSpecific primers were used to amplify the complete sequence of chitinase gene and open reading frame(ORF) from genomic DNA of N.rileyi Cq Strain extracted by CTAB.The fragment of ORF was linked with vector plasmid pPIC9K to const...
%K Keyword: Nomuraea rileyi
%K chitinase
%K clone
%K expression
%K product activity<br>关键词：莱氏野村菌
%K 几丁质酶
%K 克隆
%K 表达
%K 产物活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=E77D324B890CA7E91DB430CB934A6AA9&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=E158A972A605785F&sid=8C267C8DC97FEEEF&eid=A03A15CF5604A8B0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=0