THE RESPONSITY OF RECOMBINANT ESCHERICHIA COLI GROWTH AND PRODUCTION ON THE ACETIC ACID ACCUMULATION IN CULTURE MEDIA
乙酸积累对基因工程菌培养的影响及与培养基pH的关系

Fermentation studies were performed on an Escherichia coli culture that carries a recombinant plasmid composed of an ampicillin-resistante gene, a temperature-regulated PLPR promoter, and an interleukin-2 (IL-2) gene. The objective was to find the way to decrease the inhibition of acetic acid which was found accumulating in the media in high-cell-density culture. The responsity of the inhibition on the media pH was studied. Theresults suggested the inhibition could be decreased by regulation the media pH appropriately. In high-cell-density culture, the specific expression level and cell density was increased by raise the media pH, although there was much acetic acid accumulation too.