%0 Journal Article
%T THE RESPONSITY OF RECOMBINANT ESCHERICHIA COLI GROWTH AND PRODUCTION ON THE ACETIC ACID ACCUMULATION IN CULTURE MEDIA<br>乙酸积累对基因工程菌培养的影响及与培养基pH的关系
%A Wu Jun Yu Gongyi Feng Erling
%A <br>吴军
%A 于公义
%A 冯尔玲
%J 微生物学报
%D 1996
%I 
%X Fermentation studies were performed on an Escherichia coli culture that carries a recombinant plasmid composed of an ampicillin-resistante gene, a temperature-regulated PLPR promoter, and an interleukin-2 (IL-2) gene. The objective was to find the way to decrease the inhibition of acetic acid which was found accumulating in the media in high-cell-density culture. The responsity of the inhibition on the media pH was studied. Theresults suggested the inhibition could be decreased by regulation the media pH appropriately. In high-cell-density culture, the specific expression level and cell density was increased by raise the media pH, although there was much acetic acid accumulation too.
%K Recombinant E
%K coli
%K rIL-2
%K Fermentation
%K Acetic acid
%K Media pH<br>工程菌
%K rIL-2
%K 发酵
%K 乙酸
%K 培养基pH
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6D70FC6F56196D6883E97ACEF047C4B6&yid=8A15F8B0AA0E5323&vid=933658645952ED9F&iid=B31275AF3241DB2D&sid=DDEED1BDDBFAA8A7&eid=B40AD8FE6FA88DE9&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=0