Cloning and characterization of a novel gene cry9Ea7 encoding crystal protein with high toxicity against Trichoplusia ni
对粉纹夜蛾高毒力cry9Ea基因的克隆及表达

Objective]We cloned a novel cry9Ea gene encoding a Cry9Ea protein with a high insecticidal activity against Trichoplusia ni.Methods]We identified a cry9-type gene from Bt strain (Bt4) by PCR-RFLP.The full length cry9Ea gene was amplified by PCR with a pair of primers F9EA/R9EA.Results]We cloned the complete cry9Ea7 gene into pET21b.The SDS-PAGE results showed that the 130 kDa Cry9Ea7 protein was expressed in E.coli BL21(DE3).We also constructed an engineering strain BioHD9Ea7 by transforming a shuttle ve...