%0 Journal Article
%T Cloning and characterization of a novel gene cry9Ea7 encoding crystal protein with high toxicity against Trichoplusia ni<br>对粉纹夜蛾高毒力cry9Ea基因的克隆及表达
%A Yongxiang Sun
%A Tinghui Liu
%A Wei Guo
%A Liguang Wang
%A Weiming Sun
%A <br>孙永祥
%A 刘廷辉
%A 郭巍
%A 王立光
%A 孙伟明
%J 微生物学报
%D 2010
%I 
%X Objective]We cloned a novel cry9Ea gene encoding a Cry9Ea protein with a high insecticidal activity against Trichoplusia ni.Methods]We identified a cry9-type gene from Bt strain (Bt4) by PCR-RFLP.The full length cry9Ea gene was amplified by PCR with a pair of primers F9EA/R9EA.Results]We cloned the complete cry9Ea7 gene into pET21b.The SDS-PAGE results showed that the 130 kDa Cry9Ea7 protein was expressed in E.coli BL21(DE3).We also constructed an engineering strain BioHD9Ea7 by transforming a shuttle ve...
%K Keyword: Bacillus thuringiensis
%K cry9Ea7 gene
%K gene cloning
%K gene expression
%K insecticidal activity<br>关键词：苏云金杆菌
%K cry9Ea7基因
%K 基因克隆
%K 基因表达
%K 杀虫活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=285DC7D7E64C2F3B638F5A98120A09EE&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=94C357A881DFC066&sid=ED9DF3402785F68D&eid=10A39635766FF5D0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0