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OALib Journal期刊
ISSN: 2333-9721
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Construction of a genetically engineered and stable strain of degrading γ-hexachlorocyclohexane and carbendazim by transposon mini-Tn5
遗传稳定型六六六、多菌灵降解基因工程菌构建(英文)

Keywords: dehydrochlorinase gene linA,transposon vector pUT/mini-Tn5,multifunctional genetically engineered strain
脱氯化氢酶基因linA
,转座载体pUT/mini-Tn5,多功能工程菌,dehydrochlorinase,gene,linA,transposon,vector,pUT/mini-Tn5,multifunctional,genetically,engineered,strain,遗传,稳定型,六六六,多菌灵,降解基因,工程菌构建,strain,stable,Construction,carbendazim,plating,media,concentration,initial,activity,strong,simultaneously,multifunctional,conjugation,integrated

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Abstract:

The complete dehydrochlorinase gene linA of a gamma-hexachlorocyclohexane (gamma-HCH) degrading strain Sphingomonas sp. BHC-A, containing promoter and Shine-Dalgarno sequence (SD sequence), was amplified by PCR. The linA gene was inserted into NotI-cut transposon vector pUT/mini-Tn5 (Km(r)) to get a novel transposon vector pUT/mini-Tn5-linA. With the helper plasmid RK600, the transposon vector pUT/mini-Tn5-linA was introduced into one carbendazim degrading gram-positive strain Rhodococcus sp. DJL-6 by triparental conjugation and then the dehydrochlorinase gene linA was integrated into the chromosome of Rhodococcus sp. DJL-6 by the transposon mini-Tn5. The selected multifunctional genetically engineered strain DJL-6A could degrade gamma-HCH and carbendazim simultaneously. The dehydrochlorinase activity of DJL-6A was as strong as that of Sphingomonas sp. BHC-A in 0.05 and 5 microg/mL initial gamma-HCH concentration. The linA of the strain DJL-6A was genetically stabile after successive plating DJL-6A for 30 days on nonselective media.

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