%0 Journal Article
%T Construction of a genetically engineered and stable strain of degrading γ-hexachlorocyclohexane and carbendazim by transposon mini-Tn5<br>遗传稳定型六六六、多菌灵降解基因工程菌构建（英文）
%A Jun Wu
%A Jingliang Xu
%A Shunpeng Li
%A <br>武俊
%A 许敬亮
%A 李顺鹏等
%J 微生物学报
%D 2008
%I 
%X The complete dehydrochlorinase gene linA of a gamma-hexachlorocyclohexane (gamma-HCH) degrading strain Sphingomonas sp. BHC-A, containing promoter and Shine-Dalgarno sequence (SD sequence), was amplified by PCR. The linA gene was inserted into NotI-cut transposon vector pUT/mini-Tn5 (Km(r)) to get a novel transposon vector pUT/mini-Tn5-linA. With the helper plasmid RK600, the transposon vector pUT/mini-Tn5-linA was introduced into one carbendazim degrading gram-positive strain Rhodococcus sp. DJL-6 by triparental conjugation and then the dehydrochlorinase gene linA was integrated into the chromosome of Rhodococcus sp. DJL-6 by the transposon mini-Tn5. The selected multifunctional genetically engineered strain DJL-6A could degrade gamma-HCH and carbendazim simultaneously. The dehydrochlorinase activity of DJL-6A was as strong as that of Sphingomonas sp. BHC-A in 0.05 and 5 microg/mL initial gamma-HCH concentration. The linA of the strain DJL-6A was genetically stabile after successive plating DJL-6A for 30 days on nonselective media.
%K dehydrochlorinase gene linA
%K transposon vector pUT/mini-Tn5
%K multifunctional genetically engineered strain<br>脱氯化氢酶基因linA
%K 转座载体pUT/mini-Tn5
%K 多功能工程菌
%K dehydrochlorinase
%K gene
%K linA
%K transposon
%K vector
%K pUT/mini-Tn5
%K multifunctional
%K genetically
%K engineered
%K strain
%K 遗传
%K 稳定型
%K 六六六
%K 多菌灵
%K 降解基因
%K 工程菌构建
%K strain
%K stable
%K Construction
%K carbendazim
%K plating
%K media
%K concentration
%K initial
%K activity
%K strong
%K simultaneously
%K multifunctional
%K conjugation
%K integrated
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FDB4A2B09EBBD8300EC06A199AD939F2&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=CA4FD0336C81A37A&sid=94E7F66E6C42FA23&eid=771152D1ADC1C0EB&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13