Molecular detection of Penicillium griseofulvum as the coastal pollution indicator
近岸污染指示半知菌灰黄青霉（Penicillium griseofulvum）的分子检测

Objective] PCR method was used to detect Penicillium griseofulvum,a dominant species in marine contaminated sediments and thereby to deduce the contamination degree. Methods] According to differences in internal transcribed space (ITS) sequences of Penicillium genus and specific IAO sequence,we designed species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum and established the corresponding PCR systems. By using PCR and nested-PCR,the detection sensitivity was compared. Results] The p...