%0 Journal Article
%T Molecular detection of Penicillium griseofulvum as the coastal pollution indicator<br>近岸污染指示半知菌灰黄青霉（Penicillium griseofulvum）的分子检测
%A Shumeng Bai
%A Li Tian?
%A Zhengping Shi
%A Ning Liu
%A Jiuming Zhang
%A <br>白树猛
%A 田黎
%A 史振平
%A 刘宁
%A 张久明
%J 微生物学报
%D 2010
%I 
%X Objective] PCR method was used to detect Penicillium griseofulvum,a dominant species in marine contaminated sediments and thereby to deduce the contamination degree. Methods] According to differences in internal transcribed space (ITS) sequences of Penicillium genus and specific IAO sequence,we designed species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum and established the corresponding PCR systems. By using PCR and nested-PCR,the detection sensitivity was compared. Results] The p...
%K Keywords: coastal pollution
%K Penicillium griseofulvum
%K molecular detection<br>关键词：近海污染
%K 灰黄青霉
%K 分子检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B401AA7649B73E98A744B9475140F24F&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=CA4FD0336C81A37A&sid=228A710F49B6CE58&eid=E203FB1A272C9DD2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0