PRODUCTION CONDITIONS AND AFFINITY CHROMATOGRAPHY OF THERMOSTABLE NEUTRAL PROTEASE FROM BACILLUS STEAROTHERMOPHILUS HY-69
耐热中性蛋白酶产生条件及酶的亲和层析纯化

Strain HY-69, isolated from the springs of Xishuangbanna Autonomy, Yunnan Province, can produce thermostable neutral protease. The strain is Bacillus stearothermophilus. The production conditions of strain HY-69 have been studied. The crude enzyme, treated with ultrafiltration concentration and ammonium sulfate fractionation, was purified with Carbobenzoxy-L-phenylalanine-triethylenetetramine-sepharose 4B and Carbobenzoxy-D-phenylalanine-triethylenetetramine-sepharose 4B, repectively. Differences between two kinds of affinity chromatography have been compared. The purified enzyme appears homologeous on PAGE and SDS-PAGE.