%0 Journal Article
%T PRODUCTION CONDITIONS AND AFFINITY CHROMATOGRAPHY OF THERMOSTABLE NEUTRAL PROTEASE FROM BACILLUS STEAROTHERMOPHILUS HY-69<br>耐热中性蛋白酶产生条件及酶的亲和层析纯化
%A Jin Cheng Yang Shoujun Liu Hongdi Zhang Shuzheng
%A <br>金城
%A 杨寿钧
%A 刘宏迪
%A 张树政
%J 微生物学报
%D 1994
%I 
%X Strain HY-69, isolated from the springs of Xishuangbanna Autonomy, Yunnan Province, can produce thermostable neutral protease. The strain is Bacillus stearothermophilus. The production conditions of strain HY-69 have been studied. The crude enzyme, treated with ultrafiltration concentration and ammonium sulfate fractionation, was purified with Carbobenzoxy-L-phenylalanine-triethylenetetramine-sepharose 4B and Carbobenzoxy-D-phenylalanine-triethylenetetramine-sepharose 4B, repectively. Differences between two kinds of affinity chromatography have been compared. The purified enzyme appears homologeous on PAGE and SDS-PAGE.
%K Bacillus stearothermophilus
%K Thermostable neutral protease
%K Affinity chromatography<br>嗜热脂肪芽孢杆菌
%K 耐热中性蛋白酶
%K 亲和层析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C8FCBA0E1B564FB3748C535C72968F98&yid=3EBE383EEA0A6494&vid=339D79302DF62549&iid=E158A972A605785F&sid=B8F8200D88DDC7D6&eid=8BB50A069C48D50B&journal_id=0001-6209&journal_name=微生物学报&referenced_num=7&reference_num=2