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OALib Journal期刊
ISSN: 2333-9721
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Interaction between chromosome-encoded toxin Slr0664 and antitoxin Slr1114 of Cyanobacteria Synechocystis sp.PCC6803
蓝细菌PCC6803染色体上的毒素蛋白Slr0664与抗毒素蛋白Ssr1114的相互作用

Keywords: Keywords : Synechocystis sp,PCC6803,TA system,slr0664/ssr1114,interaction
关键词:蓝细菌PCC6803
,TA系统,ssr1114/slr0664,相互作用

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Abstract:

Abstract: Objective] We identified the interaction between toxin Slr0664 and antitoxin Slr1114, encoded by ssr1114/slr0664 system in the chromosome of cyanobacteria Synechocystis sp. PCC6803. Methods] We constructed a recombinant plasmid in which only H6-Ssr1114 was induced to express, and another plasmid in which both H6-Ssr1114 and Slr0664 was co-expressed in E. coli Bl21(DE3). After induction, we used affinity capture technique to purify H6-Ssr1114 and copurified H6-Ssr1114 and Slr0664 under different conditions. We confirmed the co-purified H6-Ssr1114 and Slr0664 by using mass spectrographic analysis. Results] When induced to express, Slr0664 showed cell toxicity leading to cell growth suppression or death. However, cells could grow normally if both H6-Ssr1114 and Slr0664 were induced to co-express. We could purify both H6-Ssr1114 and Slr0664 by His-Bind under native conditions, but only H6-Ssr1114 could be purified under denature conditions. The results of mass spectrometric analysis showed that the copurified proteins were H6-Ssr1114 and Slr0664. Conclusion] The antitoxin Slr1114 and toxin Slr0664 in ssr1114/slr0664 TA system was interacted with each other.

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