%0 Journal Article
%T Interaction between chromosome-encoded toxin Slr0664 and antitoxin Slr1114 of Cyanobacteria Synechocystis sp.PCC6803<br>蓝细菌PCC6803染色体上的毒素蛋白Slr0664与抗毒素蛋白Ssr1114的相互作用
%A Sen Ye
%A Degang Ning
%A <br>叶森
%A 宁德刚
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] We identified the interaction between toxin Slr0664 and antitoxin Slr1114, encoded by ssr1114/slr0664 system in the chromosome of cyanobacteria Synechocystis sp. PCC6803. Methods] We constructed a recombinant plasmid in which only H6-Ssr1114 was induced to express, and another plasmid in which both H6-Ssr1114 and Slr0664 was co-expressed in E. coli Bl21(DE3). After induction, we used affinity capture technique to purify H6-Ssr1114 and copurified H6-Ssr1114 and Slr0664 under different conditions. We confirmed the co-purified H6-Ssr1114 and Slr0664 by using mass spectrographic analysis. Results] When induced to express, Slr0664 showed cell toxicity leading to cell growth suppression or death. However, cells could grow normally if both H6-Ssr1114 and Slr0664 were induced to co-express. We could purify both H6-Ssr1114 and Slr0664 by His-Bind under native conditions, but only H6-Ssr1114 could be purified under denature conditions. The results of mass spectrometric analysis showed that the copurified proteins were H6-Ssr1114 and Slr0664. Conclusion] The antitoxin Slr1114 and toxin Slr0664 in ssr1114/slr0664 TA system was interacted with each other.
%K Keywords : Synechocystis sp
%K PCC6803
%K TA system
%K slr0664/ssr1114
%K interaction<br>关键词:蓝细菌PCC6803
%K TA系统
%K ssr1114/slr0664
%K 相互作用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6518A69CCC2488FB38DFD006B663100D&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=B31275AF3241DB2D&sid=6EDA906E07280FB0&eid=0B9C8D3D9D6254B2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0