Screening and breeding of high lysine-producing strains by genome shuffling
用基因组重排技术选育赖氨酸高产菌株

Abstract: Objective] To screen and breed high lysine-producing strains by genome shuffling. Methods] Corynebacterium pekinense 1 was used as the starting strain in this study. High lysine-producing strains were screened by genome shuffling. Results] Five hereditarily stable strains with high lysine production were obtained by four cycles of genome shuffling. A high lysine producing strain, F4-36, was obtained, which produced 16.95 g/dL lysine, 37.1% higher than that of the starting strain. Conclusion] Genome shuffling can be an efficient tool to screen and breed high lysine production strains.