Cloning and expression of a lipase gene in Bacillus subtilis
拆分获得(S)-酮基布洛芬脂肪酶基因在枯草芽孢杆菌中的克隆与表达

Objective]We screened a new lipase gene to asymmetricly hydrolyze the rac-ketoprofen Chloroethyl ester,to construct a secretive expression strain and to improve its enantioselectivity.Method]Strain NK13 was screened for asymmetricly hydrolyzing the rac-ketoprofen Chloroethyl ester.We prepared NK13 chromosomal DNA library to screen the lipase gene.An inducible secretion vector (pHY300-plk-sacR-gene) was developed based on the sequence that was ligated by the lipase gene and the regulatory region and the si...