%0 Journal Article
%T Cloning and expression of a lipase gene in Bacillus subtilis<br>拆分获得(S)-酮基布洛芬脂肪酶基因在枯草芽孢杆菌中的克隆与表达
%A Yunying Zhao
%A Ruien Liu
%A Lijuan Xu
%A Feifei Shi
%A Yuhong Zhao
%A Shanwei Wang
%A Jinhong Zhang
%A <br>赵运英
%A 刘瑞恩
%A 许丽娟
%A 史斐斐
%A 赵玉红
%A 王善韦
%A 张金红
%J 微生物学报
%D 2010
%I 
%X Objective]We screened a new lipase gene to asymmetricly hydrolyze the rac-ketoprofen Chloroethyl ester,to construct a secretive expression strain and to improve its enantioselectivity.Method]Strain NK13 was screened for asymmetricly hydrolyzing the rac-ketoprofen Chloroethyl ester.We prepared NK13 chromosomal DNA library to screen the lipase gene.An inducible secretion vector (pHY300-plk-sacR-gene) was developed based on the sequence that was ligated by the lipase gene and the regulatory region and the si...
%K Keywords: lipase
%K inducible expression
%K enantioselectivity
%K (S)-ketoprofen<br>关键词：脂肪酶
%K 诱导表达
%K 手性拆分
%K (S)-酮基布洛芬
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FA1C13AB3876315100CEC68D69D531E2&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=94C357A881DFC066&sid=20ED669EB429E15C&eid=DB7B2C790D19BE6E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0