Cloning and Expression of The Cadmium Resistant Determinant czcC in Escherichia coli
真养雷氏菌DKC1菌株镉抗性czcC基因的克隆与表达

The full length czcC gene encoding the structure modified protein in cadmium resistance system of Ralstonia eutropha was amplified by PCR and cloned into prokaryotic pGEM T easy,the recombinant plasmid was constructed. The sequencing results showed that there was the comparability about 98% between the sequence from the strain and the same gene accessed on the GenBank database. Then, it was successfully expressed in the E.coli BL21. The work will be available in further studies on cadmium resistance of microorganism and the design of gene engineering bacteria which are able to live in environments contaminated with higher concentration of cadmium.