%0 Journal Article
%T Cloning and Expression of The Cadmium Resistant Determinant czcC in Escherichia coli<br>真养雷氏菌DKC1菌株镉抗性czcC基因的克隆与表达
%A DUAN Xue Jun
%A MIN Hang LU Wen Ping XIA Ying
%A <br>段学军
%A 闵航
%A 吕文平
%A 夏颖
%J 微生物学报
%D 2004
%I 
%X The full length czcC gene encoding the structure modified protein in cadmium resistance system of Ralstonia eutropha was amplified by PCR and cloned into prokaryotic pGEM T easy,the recombinant plasmid was constructed. The sequencing results showed that there was the comparability about 98% between the sequence from the strain and the same gene accessed on the GenBank database. Then, it was successfully expressed in the E.coli BL21. The work will be available in further studies on cadmium resistance of microorganism and the design of gene engineering bacteria which are able to live in environments contaminated with higher concentration of cadmium.
%K Ralstonia eutropha
%K czcC
%K Cloning and Expression<br>真养雷氏菌
%K czcC
%K 克隆与表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B92AA98C54DE7BEF&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=E158A972A605785F&sid=FCACFF68346F8D4F&eid=1A033C02510EFBE6&journal_id=0001-6209&journal_name=微生物学报&referenced_num=3&reference_num=16