Determination of Coxiella burnetii in egg yolk by PCR method
PCR法对鸡卵黄中Coxiella burnetii菌的测定

Objective] Improve the detection rate of Coxiella burnetii(C.b) Com1 gene by quantitative PCR and nested PCR;determine the food safety of egg yolk by Coxiella burnetii Com1 gene detection,which has great magnificence for Coxiella burnetii epidemiology.Methods] Extract DNA in chicken eggs,determine the Com1 gene by quantitative PCR and nested PCR,and sequence the PCR products.Observe the microorganism in chicken blood leukocytes by indirect immunofluorescence.Results] Results showed that over 4 Com1 genes were detected by quantitative PCR and nested PCR,and the amount of Com1 genes in chicken eggs reached 104-106 with the positive rate of 5%-22%.The sequencing results of PCR products showed that there were variant strains in positive chicken eggs.Immunofluorescence observed the microorganism Coxiella burnetii in chicken eggs.Conclusion] Therefore we draw the conclusion that the microorganism Coxiella burnetii exists in chicken eggs and it may be the source of infection of Q fever.