%0 Journal Article
%T Determination of Coxiella burnetii in egg yolk by PCR method<br>PCR法对鸡卵黄中Coxiella burnetii菌的测定
%A QIAO Ying
%A CAI Xin-Ze
%A ZHAO Lian-Shuang
%A CHEN Dong
%A LIU Ying
%A <br>乔莹
%A 蔡鑫泽
%A 赵连爽
%A 陈冬
%A 刘颖
%J 微生物学通报
%D 2012
%I 
%X Objective] Improve the detection rate of Coxiella burnetii(C.b) Com1 gene by quantitative PCR and nested PCR;determine the food safety of egg yolk by Coxiella burnetii Com1 gene detection,which has great magnificence for Coxiella burnetii epidemiology.Methods] Extract DNA in chicken eggs,determine the Com1 gene by quantitative PCR and nested PCR,and sequence the PCR products.Observe the microorganism in chicken blood leukocytes by indirect immunofluorescence.Results] Results showed that over 4 Com1 genes were detected by quantitative PCR and nested PCR,and the amount of Com1 genes in chicken eggs reached 104-106 with the positive rate of 5%-22%.The sequencing results of PCR products showed that there were variant strains in positive chicken eggs.Immunofluorescence observed the microorganism Coxiella burnetii in chicken eggs.Conclusion] Therefore we draw the conclusion that the microorganism Coxiella burnetii exists in chicken eggs and it may be the source of infection of Q fever.
%K Coxiella burnetii
%K Quantitative PCR
%K Nested PCR
%K Chicken egg<br>Coxiella
%K burnetii
%K 定量PCR
%K 巢式PCR
%K 鸡卵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3CFA6944303655A892DF246FC3E25217&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=DF92D298D3FF1E6E&sid=5944FBFEFE5FF309&eid=226371722C2A9E20&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0