solation, screening and phylogenetic analysis of plant growth-promoting rhizobacteria (PGPR) containing ACC deaminase from Jatropha curcas L.
具有ACC脱氨酶活性的麻疯树根际促生菌(PGPR)的分离筛选及系统发育分析

Objective] In order to obtain plant growth promoting strains of production 1-aminocyclopropane-1-carboxylic acid(ACC) deaminase,nitrogen fixation,phosphate solubi-lization,and production of indoleacetic acid and siderophore.Methods] 98 rhizobacteria from Jatropha curcas L.were isolated by the method of grinding separation and 28 isolates were selected for the detection of plant growth promoting index of production ACC deaminase,ni-trogen fixation,phosphate solubilization,and production of indoleacetic acid and siderophore.Results] The results showed that 46% of the isolates produced ACC with the maximum ACC deaminase was 128.308 μmol α-KA/(mg·h),68% of the isolates produced IAA,54% had ni-trogen fixation ability and 32% of them had phosphate solubilization ability.Some of the rep-resentative isolates were seleceted for the 16S rRNA gene sequencing and analyzing.The rep-resentative strains belonged to 8 different genera including Bacillus,Arthrobacter,Pseudo-monas and Alcaligenes,with Bacillus as the predominant genus(50%).Phylogenetic analysis demonstrated that strain KLBMP 4817、KLBMP 4821 and KLBMP 4824 could be potential novel species of the genera Stenotrophomonas and Paenibacillus.Conclusion] The rhizobac-teria of Jatropha curcas L.in Panzhihua City contain a variety of bacteria as well as source of new taxa.Some of them had the ability of plant growth promoting.Among them,strain KLBMP 4804(Bacillus) had the strongest ACC deaminase activity.Strain KLBMP 4820(Ba-cillus) had the strongest ability of IAA production respectively.