%0 Journal Article %T solation, screening and phylogenetic analysis of plant growth-promoting rhizobacteria (PGPR) containing ACC deaminase from Jatropha curcas L.
具有ACC脱氨酶活性的麻疯树根际促生菌(PGPR)的分离筛选及系统发育分析 %A ZHANG Yue-Ji %A QIN Sheng %A BIAN Guang-Kai %A FEI Shi-Ming %A LI Qin %A CAO Cheng-Liang %A WANG Le-Hui %A JIANG Ji-Hong %A
张越己 %A 秦盛 %A 卞光凯 %A 费世明 %A 李钦 %A 曹成亮 %A 王乐辉 %A 蒋继宏 %J 微生物学通报 %D 2012 %I %X Objective] In order to obtain plant growth promoting strains of production 1-aminocyclopropane-1-carboxylic acid(ACC) deaminase,nitrogen fixation,phosphate solubi-lization,and production of indoleacetic acid and siderophore.Methods] 98 rhizobacteria from Jatropha curcas L.were isolated by the method of grinding separation and 28 isolates were selected for the detection of plant growth promoting index of production ACC deaminase,ni-trogen fixation,phosphate solubilization,and production of indoleacetic acid and siderophore.Results] The results showed that 46% of the isolates produced ACC with the maximum ACC deaminase was 128.308 μmol α-KA/(mg·h),68% of the isolates produced IAA,54% had ni-trogen fixation ability and 32% of them had phosphate solubilization ability.Some of the rep-resentative isolates were seleceted for the 16S rRNA gene sequencing and analyzing.The rep-resentative strains belonged to 8 different genera including Bacillus,Arthrobacter,Pseudo-monas and Alcaligenes,with Bacillus as the predominant genus(50%).Phylogenetic analysis demonstrated that strain KLBMP 4817、KLBMP 4821 and KLBMP 4824 could be potential novel species of the genera Stenotrophomonas and Paenibacillus.Conclusion] The rhizobac-teria of Jatropha curcas L.in Panzhihua City contain a variety of bacteria as well as source of new taxa.Some of them had the ability of plant growth promoting.Among them,strain KLBMP 4804(Bacillus) had the strongest ACC deaminase activity.Strain KLBMP 4820(Ba-cillus) had the strongest ability of IAA production respectively. %K ACC deaminase %K Jatropha curcas L %K PGPR %K Growth promoting %K Phylogenetic analysis
ACC脱氨酶 %K 麻疯树 %K PGPR %K 促生 %K 系统发育分析 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=D8F424933EF6BDDE61E647F481897D80&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=DF92D298D3FF1E6E&sid=859837B82DF7383D&eid=6575F6659CF52298&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0