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OALib Journal期刊
ISSN: 2333-9721
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Screening for the Peptides Blocking Cholera Phage VP1 Transfection to Host Cell by Phage Random Amino Acid Peptide Library
应用噬菌体肽库筛选能封阻霍乱噬菌体VP1转染菌细胞的寡肽

Keywords: Phage random peptide library,Typing Phage,Inactivation experiment
噬菌体随机肽库
,分型噬菌体,钝化实验

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Abstract:

Use Vibrio cholerae El Tor Typing Phage VP1 as ligand to screen phage-display random 7 amino acid peptide library, ELISA and inactivation experiment were used to identify positive clone. The ratio of output to input was increased after three rounds of screening. Pseudo-positive was decreased stepwise. It indicated the efficient enrichment. After three rounds of screening, 312 out of 360 phage clones were positive in ELISA, 1 clone shows blocking VP1 adsorbs to Vibrio cholerae by inactivation experiment. Sequencing result indicate that amino acid sequences are p245: LeuGlnGlnLysHisLeuLeu; p40,p55: GlnLeuIleMetIleArgHis; p69,p274 IleThrProArgAsnArgSer. Getting some peptides can mimick the VP1 receptor epitopes, one peptide can block VP1 transfection to host cell, it was a clue to study receptor's structure and phage infectious mechanism to host cell.

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