%0 Journal Article
%T Screening for the Peptides Blocking Cholera Phage VP1 Transfection to Host Cell by Phage Random Amino Acid Peptide Library<br>应用噬菌体肽库筛选能封阻霍乱噬菌体VP1转染菌细胞的寡肽
%A Wang Duochun Kan Biao
%A Gao Shouyi Liu Yanqing
%A <br>王多春
%A 阚飙
%A 高守一
%A 刘延清
%J 微生物学报
%D 2003
%I 
%X Use Vibrio cholerae El Tor Typing Phage VP1 as ligand to screen phage-display random 7 amino acid peptide library, ELISA and inactivation experiment were used to identify positive clone. The ratio of output to input was increased after three rounds of screening. Pseudo-positive was decreased stepwise. It indicated the efficient enrichment. After three rounds of screening, 312 out of 360 phage clones were positive in ELISA, 1 clone shows blocking VP1 adsorbs to Vibrio cholerae by inactivation experiment. Sequencing result indicate that amino acid sequences are p245: LeuGlnGlnLysHisLeuLeu; p40,p55: GlnLeuIleMetIleArgHis; p69,p274 IleThrProArgAsnArgSer. Getting some peptides can mimick the VP1 receptor epitopes, one peptide can block VP1 transfection to host cell, it was a clue to study receptor's structure and phage infectious mechanism to host cell.
%K Phage random peptide library
%K Typing Phage
%K Inactivation experiment<br>噬菌体随机肽库
%K 分型噬菌体
%K 钝化实验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1C8AB1720E001B6C&yid=D43C4A19B2EE3C0A&vid=BE33CC7147FEFCA4&iid=E158A972A605785F&sid=4ECB3941871FD391&eid=F9A6B6F259CE5121&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=7