Preparation of anti-zearalenone monoclonal antibodies and development of an indirect competitive ELISA for zearalenone
玉米赤霉烯酮单克隆抗体的制备及间接竞争ELISA检测方法的建立

Zearalenone(ZEN) is a high toxic mycotoxin.How to detect ZEN from food and feed is important and challenging.Monoclonal antibody was prepared by means of immunization in mice with conjugates of ZEN and bovine serum albumin(BSA),and enzyme-linked immunosorbent assay for ZEN based on monoclonal antibodies was developed.Four hybridoma cell strains secreting anti-zearalenone monoclonal antibodies were cloned.The subclasses of the monoclonal antibody were characterized as Ig G1 for three and Ig G2b for one.One o...