%0 Journal Article
%T Preparation of anti-zearalenone monoclonal antibodies and development of an indirect competitive ELISA for zearalenone<br>玉米赤霉烯酮单克隆抗体的制备及间接竞争ELISA检测方法的建立
%A WANG Yuan-Kai
%A WANG Jun
%A WANG Yu-Chen
%A CHEN Zhi-Fei
%A YAN Ya-Xian
%A Hao Qian-Wen
%A LI Shu-Qing
%A YU Cui
%A YANG Cui-Yun
%A SUN Jian-He
%A <br>王元凯
%A 王君
%A 王雨晨
%A 陈志飞
%A 严亚贤
%A 郝倩雯
%A 李树清
%A 于翠
%A 杨翠云
%A 孙建和
%J 微生物学通报
%D 2011
%I 
%X Zearalenone(ZEN) is a high toxic mycotoxin.How to detect ZEN from food and feed is important and challenging.Monoclonal antibody was prepared by means of immunization in mice with conjugates of ZEN and bovine serum albumin(BSA),and enzyme-linked immunosorbent assay for ZEN based on monoclonal antibodies was developed.Four hybridoma cell strains secreting anti-zearalenone monoclonal antibodies were cloned.The subclasses of the monoclonal antibody were characterized as Ig G1 for three and Ig G2b for one.One o...
%K Zearalenone
%K Monoclonal antibody
%K Enzyme-linked immunosorbent assay
%K Recovery rate<br>玉米赤霉烯酮
%K 单克隆抗体
%K 酶联免疫法
%K 回收率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=8AC6258FD9B9B7016C9401EDD7A23AF9&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=59906B3B2830C2C5&sid=3F94E028184E4B65&eid=ABD3F52B9C9810A2&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=18