Effects of H2 O2 Addition on Cell Growth and Product Formation in Long-chain Dicarboxylic Acid Fermentation
二元酸发酵过程中流加H2O2对细胞色素P450酶及产物生成的影响

When Candida tropicalis was cultivated in shakig-flask with the H 2O 2 addition, DCA (Dicarboxylic Acid,DCA) concentration was increased, especially at 2mmol/L H 2O 2 concentration. The cytochrome P450 activity assays indicated that H 2O 2 addition significantly increased the activities of cytochrome P450 and DCA production positively correlated with the activities of cytochrome P450. The study on the cell growth demonstrated that the H 2O 2 addition inhibited the cell growth rate. However, the retarding effect was not irreversible since the cell growth rate could recover slowly to the original level after the H 2O 2 addition was halted. The mechanism of inducement on cytochrome P450 by H 2O 2 addition was also discussed in this article.