PURIFICATION AND DETECTION OF APPLE STEM GROOVING VIRUS
苹果茎沟病毒的提纯和检测

Apple stem grooving virus (ASGV) G-2 and TC-3 were isolated from apple, and identified by both biological and serological methods. The ASGV G-2 was purified according to the procedure including bentonite clarification, PEG-6000 precipitation, 15% sucrose cushion and 10%-40% sucrose density gradient centrifugation, etc. The purified ASGV solution gave a A260/280 ratio of 1.15. The MW of coat protein of ASGV was 31000 determined by 13% SDS-PAGE. The above-mentioned virus preparation was used to immunize rabbit. The antiserum with high titer of 1 / 64000 tested by PTA-EL1SA was produced, and it displayed the strong specificity to ASGV. The PAS-ELISA has been used successfully to detect ASGV in apple. In addition, the DAS-ELISA showed the same detection efficiency of ASGV in apple.