%0 Journal Article
%T PURIFICATION AND DETECTION OF APPLE STEM GROOVING VIRUS<br>苹果茎沟病毒的提纯和检测
%A Wang Xiaofeng Huang Yi Wang Wenhui Liu Jun
%A <br>王小凤
%A 黄谊
%A 王文慧
%A 刘骏
%J 微生物学报
%D 1997
%I 
%X Apple stem grooving virus (ASGV) G-2 and TC-3 were isolated from apple, and identified by both biological and serological methods. The ASGV G-2 was purified according to the procedure including bentonite clarification, PEG-6000 precipitation, 15% sucrose cushion and 10%-40% sucrose density gradient centrifugation, etc. The purified ASGV solution gave a A260/280 ratio of 1.15. The MW of coat protein of ASGV was 31000 determined by 13% SDS-PAGE. The above-mentioned virus preparation was used to immunize rabbit. The antiserum with high titer of 1 / 64000 tested by PTA-EL1SA was produced, and it displayed the strong specificity to ASGV. The PAS-ELISA has been used successfully to detect ASGV in apple. In addition, the DAS-ELISA showed the same detection efficiency of ASGV in apple.
%K Apple stem grooving virus
%K Purification
%K Coat protein
%K Detection<br>苹果茎沟病毒
%K 提纯
%K 外壳蛋白
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=72CD0394B7CA24DE625B2B38C59030F1&yid=5370399DC954B911&vid=42425781F0B1C26E&iid=E158A972A605785F&sid=B799C1769FCACDC8&eid=7ABC4505E3960D2B&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=3