Advance in Technique on the Development of Uncultured Microorganism
开发未可培养微生物技术的研究进展

Viable but non-culturable state in bacterial cells as received uncultured microorganism has become the fundamental research issues in medical microbiology, epidemiology, general microbial ecology and sanitation quarantine since it was put forward in 1982. When in this state, bacterium are no longer able to grow and form colonies on conventional culture media, but maintain their patheogenicity, may become latent infection escaping detection to threaten enviroment and human security. With the development of modern molecular biology technique and metagenomics, it provide new research method and opportunity for uncultured microorganism in the environment. Recently, with the application of the metagenome technique, genetic fingerprinting technical etc, it becomes more and more popular. Simultaneously, along with this state become maturity in the laboratory. It provides a new research route for development and exploitation the uncultured microorganism.