%0 Journal Article
%T Advance in Technique on the Development of Uncultured Microorganism<br>开发未可培养微生物技术的研究进展
%A SUN Xiao-Yuan
%A LI Ying
%A QIAN Ai-Dong
%A <br>孙晓媛
%A 李 影
%A 钱爱东
%J 微生物学通报
%D 2008
%I 
%X Viable but non-culturable state in bacterial cells as received uncultured microorganism has become the fundamental research issues in medical microbiology, epidemiology, general microbial ecology and sanitation quarantine since it was put forward in 1982. When in this state, bacterium are no longer able to grow and form colonies on conventional culture media, but maintain their patheogenicity, may become latent infection escaping detection to threaten enviroment and human security. With the development of modern molecular biology technique and metagenomics, it provide new research method and opportunity for uncultured microorganism in the environment. Recently, with the application of the metagenome technique, genetic fingerprinting technical etc, it becomes more and more popular. Simultaneously, along with this state become maturity in the laboratory. It provides a new research route for development and exploitation the uncultured microorganism.
%K VBNC
%K Uncultured microorganism
%K Metagenomics
%K Genetic fingerprinting technical<br>VBNC
%K 未可培养微生物
%K 宏基因组学
%K 遗传指纹图谱技术
%K 开发环境
%K 非可培养状态
%K 微生物技术
%K 研究进展
%K Microorganism
%K Development
%K Technique
%K 资源提供
%K 利用
%K 室模型
%K 实验
%K 优势
%K 显示
%K 组技术
%K 基因组学
%K 谱技术
%K 指纹图
%K 遗传
%K 研究方法
%K 生物学技术
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=A9E885867E6582B9D4E15109BC1C699E&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=94C357A881DFC066&sid=6036F34A7A643C48&eid=EAED2CA08348AD37&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=19