Isolation and Identification of an Endopyte of Paris polyphylla var. chinensis Franch
一株产重楼皂甙内生细菌的分离与鉴定

Sixteen endophytes were isolated from the hypogenous stems of Paris polyphylla var. chinensis in Sichuan. Through thin layer chromatography (TLC) with standard comparison of Paris chinensis Franch saponins, the fermentation cultures of strains marked SNUS-1 were identified to contain Paris chinensis Franch saponins or its analogue. A phylogenetic anlysis based on 16S rDNA gene sequences (about 1500bp) shows that the p-distance of the strain SNUS-1 from Pseudomonas tolassii (AF255336) and Pseudomonas tolassii (D84028) are 0 and 0.003, respectively. These three strains gather into a cluster strictly in the phylogeny tree. Strain SNUS-1 was identified an P. tolassii based on physiological, biochemical and molecular characteristics.