%0 Journal Article
%T Isolation and Identification of an Endopyte of Paris polyphylla var. chinensis Franch<br>一株产重楼皂甙内生细菌的分离与鉴定
%A ZHANG Xiao-Jie
%A ZHA Ling-Sheng
%A CHEN Xiao-Jing
%A FENG Ding-Sheng
%A WANG Yi-Ding
%A <br>张晓洁
%A 查岭生
%A 陈小静
%A 冯定胜
%A 王一丁
%J 微生物学通报
%D 2006
%I 
%X Sixteen endophytes were isolated from the hypogenous stems of Paris polyphylla var. chinensis in Sichuan. Through thin layer chromatography (TLC) with standard comparison of Paris chinensis Franch saponins, the fermentation cultures of strains marked SNUS-1 were identified to contain Paris chinensis Franch saponins or its analogue. A phylogenetic anlysis based on 16S rDNA gene sequences (about 1500bp) shows that the p-distance of the strain SNUS-1 from Pseudomonas tolassii (AF255336) and Pseudomonas tolassii (D84028) are 0 and 0.003, respectively. These three strains gather into a cluster strictly in the phylogeny tree. Strain SNUS-1 was identified an P. tolassii based on physiological, biochemical and molecular characteristics.
%K Paris polyphylla var
%K chinensis
%K Endophyte
%K Steroidal saponins
%K 16S rDNA
%K Pseudomonas<br>重楼
%K 内生菌
%K 甾体皂甙
%K 16S
%K rDNA
%K 假单孢菌属
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=1CB1F3E2407CC70A&yid=37904DC365DD7266&vid=27746BCEEE58E9DC&iid=94C357A881DFC066&sid=656F8C8401D91023&eid=7E8E8B150580E4AB&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=4&reference_num=10