Response Surface Methodology to Optimize Lipid Production Conditions by Saccharomyces cerevisiae
响应面法优化酿酒酵母产油脂条件

Response surface methodology (RSM) was employed to optimize culture medium for lipid production of Saccharomyces cerevisiae. Firstly, according to the results of mono-factors experiment, a Plackett-Burman design was used to investigate the effects of different factors in the ferment medium, three statistically significant factors are:citric acid, CaCl2, initial pH value, and then steepest ascent procedures was employed to define optimal response region for these three factors. Finally, Box-Behnken was employed to design. The results of experiments was analyzed by RSM with Minitab15.0 soft. The optimal lipid production medium is: glucose 15%, peptone 0.2%, yeast extraction 0.4%, citric acid 0.471%, MgSO4?7H2O 0.1%, ZnSO4?7H2O 0.2%, CaCl2 0.025%, FeSO4?7H2O 0.005%, initial pH value 6.74, 180 r/min, incubme in 30?C for 96 h. Lipid production (dry weight) after optimization is increased from 4.76% to 14.55%. The yield of lipid is increased by about 3 times.