%0 Journal Article
%T Response Surface Methodology to Optimize Lipid Production Conditions by Saccharomyces cerevisiae<br>响应面法优化酿酒酵母产油脂条件
%A YANG Shi-Quan ZHANG Xi-Cheng LIU Jun-Feng WANG Fang TAN Tian-Wei DENG Li
%A <br>杨实权
%A 张喜成
%A 刘军锋
%A 王芳
%A 谭天伟
%A 邓利
%J 微生物学通报
%D 2010
%I 
%X Response surface methodology (RSM) was employed to optimize culture medium for lipid production of Saccharomyces cerevisiae. Firstly, according to the results of mono-factors experiment, a Plackett-Burman design was used to investigate the effects of different factors in the ferment medium, three statistically significant factors are:citric acid, CaCl2, initial pH value, and then steepest ascent procedures was employed to define optimal response region for these three factors. Finally, Box-Behnken was employed to design. The results of experiments was analyzed by RSM with Minitab15.0 soft. The optimal lipid production medium is: glucose 15%, peptone 0.2%, yeast extraction 0.4%, citric acid 0.471%, MgSO4?7H2O 0.1%, ZnSO4?7H2O 0.2%, CaCl2 0.025%, FeSO4?7H2O 0.005%, initial pH value 6.74, 180 r/min, incubme in 30?C for 96 h. Lipid production (dry weight) after optimization is increased from 4.76% to 14.55%. The yield of lipid is increased by about 3 times.
%K Saccharomyces cerevisiae
%K Lipid
%K Response surface methodology
%K Optimization<br>酿酒酵母
%K 油脂
%K 响应面法
%K 优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=BDC53BF0117BA116656DD53A29708FBA&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=CA4FD0336C81A37A&sid=3ED24C32270473AA&eid=9AC927517513F8AB&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=6&reference_num=0