Cloning, expression and characterization of xylose isomerase, XylA from Geobacillus sp. Y565-5
地芽孢杆菌Y565-5分离鉴定及其木糖异构酶基因xylA的克隆表达和酶学性质

Xylose-utilizing and thermophilic Geobacillus sp.Y565-5 was isolated from surface soil of an oilfield in Yumen Town,Gansu Province,China.A xylose isomerase(XylA) gene was cloned from the strain by PCR.The open reading frame of xylA(1 182 bp) encoded a protein of 394 amino acids,which showed high sequence homology(99% identity) with that of Geobacillus sp.Y412MC52.The in-tact coding region was subcloned into pET28a(+) vector and expressed in Escherichia coli BL21(DE3).The molecular weight of the recombinant ...