%0 Journal Article
%T Cloning, expression and characterization of xylose isomerase, XylA from Geobacillus sp. Y565-5<br>地芽孢杆菌Y565-5分离鉴定及其木糖异构酶基因xylA的克隆表达和酶学性质
%A ZHANG Jie
%A HUANG Zhi-Yong
%A WANG Qin-Hong
%A WANG Yong-Li
%A WANG Shuo
%A <br>张洁
%A 黄志勇
%A 王钦宏
%A 王永莉
%A 王硕
%J 微生物学通报
%D 2011
%I 
%X Xylose-utilizing and thermophilic Geobacillus sp.Y565-5 was isolated from surface soil of an oilfield in Yumen Town,Gansu Province,China.A xylose isomerase(XylA) gene was cloned from the strain by PCR.The open reading frame of xylA(1 182 bp) encoded a protein of 394 amino acids,which showed high sequence homology(99% identity) with that of Geobacillus sp.Y412MC52.The in-tact coding region was subcloned into pET28a(+) vector and expressed in Escherichia coli BL21(DE3).The molecular weight of the recombinant ...
%K Geobacillus sp
%K Y565-5
%K xylose isomerase
%K cloning
%K expression<br>Geobacillus
%K sp.Y565-5
%K 木糖异构酶
%K 克隆
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=20E65ECF19352841B12D10A167BCE682&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=5D311CA918CA9A03&sid=008520E0B52E94B3&eid=B8D8B337883846D1&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15