Isolation and Genetic Diversity of the Endophytic Actinomycetes from Salvia miltiorrhiza Bge. and Polygonatum sibiricum Red.
丹参、黄精内生放线菌的分离及遗传多样性分析

Two species of Chinese medicinal plants, Salvia miltiorrhiza Bge. and Polygonatum sibiricum Red., were collected from Suining region of Sichuan province. The plants were disinfected with 0.87% hypoclorite under different time and treated with the methods of organic law and homogenate law, respectively. Then endophytes were isolated in the media HV, G2, S amended with which use different concentrations of potassium dichromate plus malidixic acid as inhibitors. The method of isolating endophytic actinomycetes from the medicinal plants was optimized. Fifty-two endophytic actinomycetes with different colony size, shape and color were isolated and purified. Twelve representative strains were chosen for genetic diversity study by 16S rRNA PCR-PFLP. Five clusters were abtained based the 16S rRNA PCR-RFLP pattens which indicated the high genetic diversity of the endophytic actinomycetes in medicinal plants.