%0 Journal Article
%T Isolation and Genetic Diversity of the Endophytic Actinomycetes from Salvia miltiorrhiza Bge. and Polygonatum sibiricum Red.<br>丹参、黄精内生放线菌的分离及遗传多样性分析
%A LI Xiao-Lin
%A YUAN Hong-Mei
%A QI Shan-Shan
%A FENG Mao-Lin
%A LIU Hong-Wei
%A ZHANG Xiao-Ping
%A <br>李小林
%A 袁红梅
%A 戚珊珊
%A 冯茂林
%A 刘洪伟
%A 张小平
%J 微生物学通报
%D 2010
%I 
%X Two species of Chinese medicinal plants, Salvia miltiorrhiza Bge. and Polygonatum sibiricum Red., were collected from Suining region of Sichuan province. The plants were disinfected with 0.87% hypoclorite under different time and treated with the methods of organic law and homogenate law, respectively. Then endophytes were isolated in the media HV, G2, S amended with which use different concentrations of potassium dichromate plus malidixic acid as inhibitors. The method of isolating endophytic actinomycetes from the medicinal plants was optimized. Fifty-two endophytic actinomycetes with different colony size, shape and color were isolated and purified. Twelve representative strains were chosen for genetic diversity study by 16S rRNA PCR-PFLP. Five clusters were abtained based the 16S rRNA PCR-RFLP pattens which indicated the high genetic diversity of the endophytic actinomycetes in medicinal plants.
%K Medicinal plant
%K Endophytic actinomycetes
%K Solation
%K Genetic diversity<br>药用植物
%K 内生放线菌
%K 分离
%K 遗传多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=C050AFDFA21BF64335FFD2750CA116BD&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=9CF7A0430CBB2DFD&sid=2AF38D6ACB828704&eid=6733A846E5AFAE2E&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0