Effect of lactobacilli on production of Th1/Th2 cytokine and antibody in lymphocyte of bovine β-lactoglobulin-sensitized mice in vitro
乳杆菌对牛乳β-乳球蛋白致敏小鼠淋巴细胞分泌Th1/Th2型细胞因子和抗体的体外影响

To investigate the effect of lactobacilli on the Th1/Th2 cytokine and antibody production in sen-sitized-mice spleen lymphocytes in vitro,BALB/c mice were intraperitoneally injected with β-lactoglobulin,After successfully established the allergic model,the sensitized splenic cells were stimulated with ac-tive/heat-killed lactobacilli(107 CFU/mL) at different species in vitro.Supernatants were collected to measure the cytokines(IL-12,IFN-γ,IL-4) and Igs(total IgE,β-Lg-specific IgE,total IgG) production by ELISA assay.In vitro studies,4 active/heat-killed lactobacilli could differently modulate the produc-tion of Th1/Th2 cytokine and antibody,especially the heat-killed Lactobacillus fermentum and Lacto-bacillus acidophilus were not inferior to the live strains.These two heat-killed Lactobacillus suppressed IL-4 and IL-10 production by stimulating IL-12 and IFN-γ production.Their IFN-γ/IL-4 value,which represents the Th1/Th2 cell balance were higher than that of other strains,and compared with the con-trol were significant(P<0.05).At the same time,these two heat-killed strains significantly decreased the secretion of total IgE,β-Lg-specific IgE and total IgG(P<0.05).These data indicate that lactobacilli may be effective in decreasing the antibody production in sensitized-mice splenocytes by increasing the ratio of IFN-γ/IL-4 and changing the dominant of Th2.Four strains may differ in their respective ability to improve Th1/Th2 cell balance toward Th1 dominance.