%0 Journal Article
%T Effect of lactobacilli on production of Th1/Th2 cytokine and antibody in lymphocyte of bovine β-lactoglobulin-sensitized mice in vitro<br>乳杆菌对牛乳β-乳球蛋白致敏小鼠淋巴细胞分泌Th1/Th2型细胞因子和抗体的体外影响
%A MA Dong-Xue
%A MENG Xiang-Chen
%A GAO Xue-Jun
%A LI Ai-Li
%A <br>马冬雪
%A 孟祥晨
%A 高学军
%A 李艾黎
%J 微生物学通报
%D 2011
%I 
%X To investigate the effect of lactobacilli on the Th1/Th2 cytokine and antibody production in sen-sitized-mice spleen lymphocytes in vitro,BALB/c mice were intraperitoneally injected with β-lactoglobulin,After successfully established the allergic model,the sensitized splenic cells were stimulated with ac-tive/heat-killed lactobacilli(107 CFU/mL) at different species in vitro.Supernatants were collected to measure the cytokines(IL-12,IFN-γ,IL-4) and Igs(total IgE,β-Lg-specific IgE,total IgG) production by ELISA assay.In vitro studies,4 active/heat-killed lactobacilli could differently modulate the produc-tion of Th1/Th2 cytokine and antibody,especially the heat-killed Lactobacillus fermentum and Lacto-bacillus acidophilus were not inferior to the live strains.These two heat-killed Lactobacillus suppressed IL-4 and IL-10 production by stimulating IL-12 and IFN-γ production.Their IFN-γ/IL-4 value,which represents the Th1/Th2 cell balance were higher than that of other strains,and compared with the con-trol were significant(P<0.05).At the same time,these two heat-killed strains significantly decreased the secretion of total IgE,β-Lg-specific IgE and total IgG(P<0.05).These data indicate that lactobacilli may be effective in decreasing the antibody production in sensitized-mice splenocytes by increasing the ratio of IFN-γ/IL-4 and changing the dominant of Th2.Four strains may differ in their respective ability to improve Th1/Th2 cell balance toward Th1 dominance.
%K Lactobacilli
%K β-Lactoglobulin
%K Allergy
%K Th1/Th2 cell balance
%K Antibody<br>乳杆菌
%K β-乳球蛋白
%K 过敏
%K Th1/Th2细胞平衡
%K 抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=C38B24F2A5403A3C4B34ECEB73E8F9AC&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=DF92D298D3FF1E6E&sid=EE05CC1F800E4629&eid=81A5772701933E75&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=22