Recombinant Expression and Antimicrobial Assay of Hevein-like Peptide AbAMP1
类橡胶蛋白AbAMP1的重组表达及其抑菌活性研究

The gene of hevein-like peptide AbAMP1 was cloned into the vector pPIC9 successfully.After linearized with restriction enzymes,the recombinant plasmid pPIC9-Ab was transformed into Pichia pastoris SMD1163 by electrotransformation.The transformants were screened on MD plate medium,and then identified using PCR method.The transformant AS16 with Mut~+ phenotype was used for inducing expression and other assays.A protein band of about 4.7 kD was detected on the gel by a tricine-SDS-PAGE analysis of induced cult...