%0 Journal Article
%T Recombinant Expression and Antimicrobial Assay of Hevein-like Peptide AbAMP1<br>类橡胶蛋白AbAMP1的重组表达及其抑菌活性研究
%A DENG Ming-Rong
%A LONG Liang-Kun
%A ZHU Hong-Hui
%A QU Xin-Yong
%A <br>邓名荣
%A 龙良鲲
%A 朱红惠
%A 曲新勇
%J 微生物学通报
%D 2008
%I 
%X The gene of hevein-like peptide AbAMP1 was cloned into the vector pPIC9 successfully.After linearized with restriction enzymes,the recombinant plasmid pPIC9-Ab was transformed into Pichia pastoris SMD1163 by electrotransformation.The transformants were screened on MD plate medium,and then identified using PCR method.The transformant AS16 with Mut~+ phenotype was used for inducing expression and other assays.A protein band of about 4.7 kD was detected on the gel by a tricine-SDS-PAGE analysis of induced cult...
%K Hevein-like peptide
%K AbAMP1
%K Recombinant expression
%K Antimicrobial assay<br>类橡胶蛋白
%K AbAMP1
%K 重组表达
%K 抑菌活性
%K 胶蛋白
%K 重组表达
%K 抑菌带
%K 活性研究
%K Peptide
%K Assay
%K Expression
%K 酵母菌
%K 丝状真菌
%K 抑制作用
%K 细菌
%K Staphylococcus
%K aureus
%K subtilis
%K Fusarium
%K oxysporum
%K 显示
%K 活性试验
%K 上清液
%K 天然活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=C738F1EFE6D26790A5C804F58E635780&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=94C357A881DFC066&eid=9CF7A0430CBB2DFD&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=12