Cloning and Functional Analysis of pyrD Gene Involved in Siderophores Biosynthesis in Pseudomonas pseudoalcaligenes B50
Pseudomonas pseudoalcaligenes B50铁载体合成相关基因pyrD的克隆与功能分析

In order to study the mechanism of bacteria producing siderophore, B50 was isolated from the cotton rhizosphere. The strain B50 can produce siderophore under iron-limiting conditions. It was identified as Pseudomonas pseudoalcaligenes based on its morphological, physical-chemical characteristics and 16S rDNA sequence. Mutants of B50 were obtained through conjugation. The pyrD gene was cloned from the mutant deficient in siderophores synthesis by TAIL-PCR (Thermal asymmetric interlaced). The pyrD gene is required for the synthesis of dihydroorotate which is a predecessor of deoxyquinoline. Deoxyquinoline was the important part of several siderophores. This is the first report that the Pseudomonas pseudoalcaligenes can produce siderophore.