%0 Journal Article
%T Cloning and Functional Analysis of pyrD Gene Involved in Siderophores Biosynthesis in Pseudomonas pseudoalcaligenes B50<br>Pseudomonas pseudoalcaligenes B50铁载体合成相关基因pyrD的克隆与功能分析
%A DING Yan-Qin
%A HUANG Wei-Hong
%A YAO Liang-Tong
%A YU Su-Fang
%A DU Bing-Hai
%A <br>丁延芹
%A 黄伟红
%A 姚良同
%A 于素芳
%A 杜秉海
%J 微生物学通报
%D 2010
%I 
%X In order to study the mechanism of bacteria producing siderophore, B50 was isolated from the cotton rhizosphere. The strain B50 can produce siderophore under iron-limiting conditions. It was identified as Pseudomonas pseudoalcaligenes based on its morphological, physical-chemical characteristics and 16S rDNA sequence. Mutants of B50 were obtained through conjugation. The pyrD gene was cloned from the mutant deficient in siderophores synthesis by TAIL-PCR (Thermal asymmetric interlaced). The pyrD gene is required for the synthesis of dihydroorotate which is a predecessor of deoxyquinoline. Deoxyquinoline was the important part of several siderophores. This is the first report that the Pseudomonas pseudoalcaligenes can produce siderophore.
%K Pseudomonas pseudoalcaligenes
%K Siderophores biosynthesis
%K pyrD<br>Pseudomonas
%K pseudoalcaligenes
%K 铁载体合成
%K pyrD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3DA263CBA9E1115322A97C15154C766E&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=DF92D298D3FF1E6E&sid=19944D0953C5A716&eid=630BDAC3CC18EDE0&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0