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OALib Journal期刊
ISSN: 2333-9721
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Isolation of a Micrococcus luteus Strain with Producing Fibrinolysin and Cloning of Fibrinolitic Enzyme Gene
纤溶酶产生菌—藤黄微球菌的筛选、鉴定及纤溶酶基因的克隆

Keywords: Fibrinolytic enzyme,Gene cloning,16S rDNA,Micrococcus luteus
纤溶酶
,基因克隆,16S,rDNA,藤黄微球菌

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Abstract:

A strain, producing a fibrinolytic enzyme, was isolated from the nature environment by using blood powder plates gathering and fibrin plates screening, and was named ML909. All results from morphological, physiological and biochemical characterization, the 16S rDNA sequence and phylogenetic assay identified that the strain belongs to Micrococcus luteus. The DNA fragment encoding mature peptide of fibrinolytic enzyme was cloned by PCR and sequenced. The gene sequence was registered in GenBank of NCBI and the accession number is EU232121.

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