BREEDING OF SINGLE COMPONENT SAGAMICIN-PRODUCING STRAIN AND STUDIES ON FERMENTATION CONDITIONS
小诺霉素单组份菌种选育及发酵条件研究

Micromonospora echinospora A-23 was screened through protoplast UV-ray, electrofasion regeneration, near ultraviolet light in presence of 8-methoxypsoralen, combined tratment of LiCl and UV-ray, etc, and isolated single colonies with natural selection. A high and stable sagamictu-producing strain MS-116 was obtained through several-decade generations of the treatment. The main component content increased to over 85%. The culture medium and the effective factors on fermentation was also studied. Trial-production by 30 ton-fermentor indicated that the strain is high and stable is high and stable sapamicin-producing strain for industrial production.