STUDES ON FUSON OF FLUORESCEIN-LABELED PROTOPLASTS FROM FLAMMULINA VELUTIPES
荧光标记金针菇原生质体融合

This paper studied the fusion of fluorescein-labeled protoplastS of two kinds ofFlommulina velutipes. The Fluorescein iso-thiocyanate (FITC)-labeled protoplasts of strain W19and the non-labeled protoplasts of strain Y7 could be induced to fuse in the presence ofpolyethylene glycol (PEG). A pair of sticked protoplasts which only one labeled could beselected direchy using whcromahipulator. We screened the fusant from the regenerated coloniesby the clamp connechon of dikaryophyte. Anlysising the esteraes isozyme, we found theisozyme bands of the fusants were complemental with those of their parentS. The growth rateof the mycelia and the biomass 0f many fusantS were superior to those of their parents.