%0 Journal Article
%T STUDES ON FUSON OF FLUORESCEIN-LABELED PROTOPLASTS FROM FLAMMULINA VELUTIPES<br>荧光标记金针菇原生质体融合
%A Cheng Yali
%A Zhu Baocheng
%A Li Liangliang
%A Yan Keqin
%A Li Qingyu
%A <br>成亚利
%A 朱宝成
%A 李亮亮
%A 燕克勤
%A 李庆余
%J 微生物学通报
%D 1997
%I 
%X This paper studied the fusion of fluorescein-labeled protoplastS of two kinds ofFlommulina velutipes. The Fluorescein iso-thiocyanate (FITC)-labeled protoplasts of strain W19and the non-labeled protoplasts of strain Y7 could be induced to fuse in the presence ofpolyethylene glycol (PEG). A pair of sticked protoplasts which only one labeled could beselected direchy using whcromahipulator. We screened the fusant from the regenerated coloniesby the clamp connechon of dikaryophyte. Anlysising the esteraes isozyme, we found theisozyme bands of the fusants were complemental with those of their parentS. The growth rateof the mycelia and the biomass 0f many fusantS were superior to those of their parents.
%K Flammulina velutipes
%K Protoplast
%K Fluorescein-labeled
%K Fusion
%K Isozyme<br>金针菇
%K 原生质体
%K 荧光标记
%K 融合
%K 同工酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=9481E982FF0CADD3813CA678C3EA25F8&yid=5370399DC954B911&vid=B91E8C6D6FE990DB&iid=B31275AF3241DB2D&sid=717CC18E05F2AFA0&eid=CF6CB42CFF3D4C4E&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=3&reference_num=2